The discovery that green fluorescent protein (GFP) from the jellyfish Aequorea victoria could be used to label proteins in cells led to a revolution in our ability to follow the behaviour of proteins in live cells and whole organisms. A bewildering range of fluorescent proteins and sensors are now available that span the fluorescent spectrum, and together these provide the opportunity to dissect cell biological processes with exquisite spatiotemporal resolution.
This poster by Michael Lin, Atsushi Miyawaki and Roger Tsien provides a user’s guide to the range of fluorescent proteins and sensors now available, and the cell biological questions to which they can be best applied.
Visit www.nature.com/nrm/posters/fluorescent to view this Poster!
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